10 Questions You Should Know about T75 Flask

10 Questions You Should Know about T75 Flask.

When it comes to cell culture, T75 flask is a commonly used tool that plays a crucial role in the growth and maintenance of cell lines. Here are 10 important questions you should know about T75 flask:

1. What is a T75 flask?

A T75 flask is a type of cell culture vessel that has a working volume of 75ml. It is specifically designed for the growth and maintenance of adherent cell lines.

2. Why is it called a T75 flask?

The "T" in T75 flask stands for tissue culture, which is the traditional term used in cell culture. The "75" denotes the working volume of the flask, which is 75ml.

3. What is the surface area of a T75 flask?

A T75 flask typically has a surface area of about 75 cm², which is ideal for the cultivation of adherent cell lines that require attachment to a solid substrate for growth.

4. How often should the media in a T75 flask be changed?

The media in a T75 flask should be changed every 2-3 days to provide the cells with fresh nutrients and remove waste products.

5. What is the optimal cell seeding density for a T75 flask?

The optimal cell seeding density for a T75 flask is usually around 5,000-10,000 cells/cm², depending on the cell line being cultured.

6. How do you prevent contamination in a T75 flask?

Contamination in a T75 flask can be prevented by maintaining aseptic techniques, using sterile reagents and equipment, and regularly checking for signs of contamination such as changes in media color or turbidity.

7. Can a T75 flask be used for cell passaging?

Yes, a T75 flask can be used for cell passaging by detaching the adherent cells with trypsin-EDTA, splitting them into new flasks, and allowing them to grow and expand.

8. What is the maximum passage number for cells in a T75 flask?

The maximum passage number for cells in a T75 flask depends on the cell line being cultured and how well it has been maintained. Generally, cells should not be passaged beyond 15-20 passages to avoid cellular senescence.

9. How can you store a T75 flask in the incubator?

To store a T75 flask in the incubator, make sure it is tightly sealed with the cap to prevent contamination and place it in a humidified atmosphere with 5% CO2 at 37°C.

10. What are the advantages of using a T75 flask for cell culture?

The T75 flask provides a convenient and cost-effective vessel for the growth and maintenance of adherent cell lines, allowing researchers to scale up their experiments and easily monitor cell behavior. Additionally, the standardization of flask size and surface area makes it easier to compare results between different experiments and laboratories.

In conclusion, understanding the basics of T75 flask usage is essential for successful cell culture experiments. By knowing the answers to these 10 questions, researchers can optimize their cell culture techniques, improve reproducibility, and ultimately advance scientific discoveries in the field of biology.

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